刘引,龚文玲,鲍五洲,郭兰萍,徐扬,刘义梅,刘大会
LIU Yin,GONG Wen-ling,BAO Wu-zhou,GUO Lan-ping,XU Yang,LIU Yi-mei,LIU Da-hui

• 1:湖北中医药大学中药资源与中药化学湖北省重点实验室
• 2:麻城市农业技术推广中心
• 3:中国中医科学院中药资源中心道地药材国家重点实验室培育基地

摘要(Abstract)：

该文采用中药色谱指纹图谱相似度评价系统,建立了湖北麻城福田河镇、黄土岗镇以及武汉三地共29批不同品种菊花的HPLC指纹图谱,并对其进行相似度评价和共有峰聚类分析;还测定比较了样品中有效成分绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸的含量。29批不同品种菊花指纹图谱共有11个共有峰,相似度为0. 802～0. 975;聚类分析将杭菊和贡菊聚为一类,黄菊单独聚为一类。所建立的指纹图谱方法可为菊花品种的鉴别和质量标准制定提供依据。29批菊花绿原酸质量分数为4. 092～11. 723 mg·g-1,木犀草苷为1. 010～11. 713 mg·g-1,3,5-O-二咖啡酰基奎宁酸为8. 828～33. 435 mg·g-1,均达到药典标准,但不同品种之间差异较大。综合菊花中3种有效成分含量和指纹图谱峰多样性来看,麻城本地福白菊这一特色种质资源的品质比较优异,生产上要加强地方特色种质资源的保护。
In this paper,the fingerprint of different varieties of chrysanthemum were established with " Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica" and the content of chlorogenic acid,galuteolin and 3,5-O-dicaffeoylquinic acid in 29 batches of different varieties of chrysanthemum in Futianhe town,Huangtugang town and Wuhan city were compared. At the same time,similarity evaluation and common peak clustering analysis were carried out. There were 11 common peaks in the fingerprints of 29 batches of different varieties of chrysanthemum,and the similarity ranged from 0. 802 to 0. 975. Hangju and Gongju were divided into one group by cluster analysis,and Huangju into another category. The established fingerprint method provides a basis for the identification of chrysanthemum cultivars. The content of 29 batches of chlorogenic acid was between 4. 092 and 11. 723 mg·g-1,luteolin was between 1. 010 and 11. 713 mg·g-1,and 3,5-O-dicaffeoylquinic acid was between 8. 828 and 33. 435 mg·g-1,both reach the pharmacopoeia standard,but the effective components of different varieties of chrysanthemum were quite different. Based on the contents of three active ingredients and the diversity of fingerprint peaks,the quality of the characteristic germplasm resource of local Fubaijuin Macheng is superior,and the protection of local characteristic germplasm resource should be strengthened in production.

关键词(KeyWords)： 菊花;福白菊;绿原酸;木犀草苷;3,5-O-二咖啡酰基奎宁酸;HPLC指纹图谱
chrysanthemum;Fubaiju;chlorogenic acid;galuteolin;3,5-O-dicaffeoylquinic acid;HPLC fingerprint

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2017FYC1700704,2018YFD0201107);; 现代农业产业技术体系建设专项(CARS-21)

作者(Author): 刘引,龚文玲,鲍五洲,郭兰萍,徐扬,刘义梅,刘大会
LIU Yin,GONG Wen-ling,BAO Wu-zhou,GUO Lan-ping,XU Yang,LIU Yi-mei,LIU Da-hui

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