叶浩婷,李晓蕾,晁志
YE Hao-ting,LI Xiao-lei,CHAO Zhi

• 1:南方医科大学中医药学院

摘要(Abstract)：

基于COⅠ序列设计中国壁虎特异性引物对GZG1/GZG2,采用荧光定量PCR技术,对金龙胶囊配方中的中国壁虎组分进行定性及定量分析。所建立的中国壁虎定量分析的标准曲线为y=-3.012 7x+34.501,其中y为Ct值,x为COⅠ片段拷贝数以10为底的对数值。对10份自制供试样品的测定中,含有中国壁虎的供试品均出现扩增,不含中国壁虎的全伪品组及空白对照组未见扩增,其中全正品组、正伪品1∶1组及正伪品1∶4组所得中国壁虎COⅠ片段拷贝数依次为11.511×10~6,6.416×10~6,2.553×10~6拷贝/μL,大致符合投料比,可为含有动物药成分的中成药提供质量控制的新思路。
A pair of species-specific primer(GZG1/GZG2) based on COⅠ sequence regions for identification of Gekko chinensis were designed. A fluorescent quantitative PCR method was established to identify and quantify G. chinensis from Jinlong Capsules Formula. A standard curve for quantitative analysis of G. chinensis was established(the standard curve equation: y=-3.012 7x + 34.501, y is Ct value, x is lg N, N is the copies of COⅠ fragment from G. chinensis). Samples included G. chinensis appeared amplification, while falsify group(not included G. chinensis) and negative control did not have amplification products. The copy number of COⅠ region of G. chinensis was respectively 11.511×10~6, 6.416×10~6,2.553×10~(6 ) copies/μL in all quality goods, quality goods-adulterants 1∶1, quality goods-adulterants 1∶4. The results accorded with proportion of adding amount roughly. This study can provide a new strategy for quality control of Chinese patent medicine containing animal drug ingredients.

关键词(KeyWords)： 金龙胶囊配方;中国壁虎;荧光定量PCR;定性;定量
Jinlong Capsules Formula;Gekko chinensis;fluorescence quantitative PCR;qualitative analysis;quantification

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81573540)

作者(Author): 叶浩婷,李晓蕾,晁志
YE Hao-ting,LI Xiao-lei,CHAO Zhi

DOI: 10.19540/j.cnki.cjcmm.2018.0121

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