DNA条形码分子鉴定在动物药中的研究进展Research progress about molecular identification of DNA barcoding in medicinal animal
荆礼,苏航,睢博文,张鹤,潘黛安,齐滨
JING Li,SU Hang,SUI Bo-wen,ZHANG He,PAN Dai-an,QI Bin
摘要(Abstract):
动物药具有疗效高、活性强、应用广、潜力大的特点,但现今市场流通的动物药混伪品种类繁多且复杂。DNA条形码分子鉴定技术是近年来新兴的分子生物技术,是传统鉴定方法强有力的补充。该技术能够很好的从分子水平上鉴别动物药基源物种,具有较高的准确性,可以对动物药进行快速鉴定,对药材市场进行有效监控。该就DNA条形码分子鉴定技术流程、近年来DNA条形码在动物药鉴定中的应用研究、DNA条形码分子鉴定技术的局限性等方面进行综述。
The use of animal medicine has a long history in China, it has the characteristics of high curative effect,strong activity, wide application and great potential. However,the circulation of animal medicine in current market mixed counterfeit variety and complex. Molecular identification technology of DNA barcoding is an emerging molecular biotechnology in recent years, it is a powerful supplement to traditional identification methods. This method can well identify animal species at the molecular level and has high accuracy, it can identify animal medicines quickly and monitor the medicine market effectively. This article summarizes the research process of molecular identification of DNA barcoding, the application of DNA barcoding in medicinal animals identification in recent years, and the limitations of DNA barcoding technology.
关键词(KeyWords):
动物药;DNA条形码;线粒体细胞色素C氧化酶亚基Ⅰ;鉴定
medicinal animals;DNA barcoding;mitochondrial cytochrome c oxidation subunit Ⅰ;identification
基金项目(Foundation): 国家中药标准化项目(ZYBZH-Y-JL-26,ZYBZH-C-JL-22);; 国家自然科学基金项目(81503243)
作者(Author):
荆礼,苏航,睢博文,张鹤,潘黛安,齐滨
JING Li,SU Hang,SUI Bo-wen,ZHANG He,PAN Dai-an,QI Bin
参考文献(References):
- [1] 高学敏.中药学 [M].北京:中国中医药出版社,2007.
- [2] 中国药典.一部[S]. 2015.
- [3] 袁庆军,张文婧, 姜丹,等.论中药分子鉴定的方法和原则[J].植物分类与资源学报,2012,34(6):607.
- [4] 陈士林, 庞晓慧,姚辉,等.中药DNA条形码鉴定体系及研究方向[J].世界科学技术——中医药现代化, 2011,13(5):747.
- [5] 王孟虎,许亮,康廷国,等.动物类中药DNA条形码鉴定研究进展[J].中国实验方剂学杂志,2016,22(15):227.
- [6] 吴绍亮, 刘晗, 邓俊花,等.动物源性中药材甄别技术研究进展[J].检验检疫学刊,2017,27(2):35.
- [7] Tautz D, Arctander P, Minelli A,et al.DNA points the way ahead in taxonomy[J].Nature,2002,418(6897):479.
- [8] Hebert P D,Cywinska A,Ball S L,et al.Biological identifications through DNA barcodes[J].Proc R Soc Lond Ser B Biol Sci,2003,270(1512):313.
- [9] Hebert P D,Ratnasingham S,Waard J R.Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species[J].Proc Biol Sci,2003,270 (S1):S96.
- [10] Taberlet P,Coissac E,Pompanon F,et al.Power and limitations of the chloroplast trnL (UAA) intron for plant DNA barcoding[J].Nucleic Acids Res,2007,35(3):e14.
- [11] Brown W M,Prager E M,Wang A,et al.Mitochondrial DNA sequences of primates: tempo and mode of evolution[J].J Mol Evol,1982,18(4):225.
- [12] 陈士林,姚辉,韩建萍,等.中药材DNA条形码分子鉴定指导原则[J].中国中药杂志,2013,38(2):141.
- [13] 石林春,姚辉,谢丽芳,等.中国动物药材DNA条形码数据库[J].中国中药杂志,2014,39(12):2155.
- [14] Chen S L,Hui Y,Han J P,et al.Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species[J].PLoS ONE,2010,5(1):e8613.
- [15] Ross H A,Murugan S,Li W L.Testing the reliability of genetic methods of species identification via simulation[J].Syst Biol,2008,57(2):216.
- [16] Kimura M.A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences[J].J Mol Evol,1980,16(2):111.
- [17] 黄勇,张月云,赵成坚,等.DNA条形码技术在常见中药材蛇类鉴别中的应用[J].中国中药杂志,2015,40(5):868.
- [18] 张改霞,刘金欣,贾静,等.基于COⅠ条形码的海马药材及其混伪品分子鉴定[J].中国药学杂志,2015,50(17):1469.
- [19] 张红印,陈俊,贾静,等.中药材蜈蚣及其混伪品DNA条形码鉴别研究[J].中国中药杂志,2014,39(12):2208.
- [20] 王孟虎,康廷国,许亮,等.基于COⅠ序列的哈蟆油基原动物DNA条形码鉴定研究[J].中国中药杂志,2017,42(8):1572.
- [21] 樊佳佳,宋明,宋驰,等.中药材蟾皮及其混伪品的DNA条形码鉴定研究[J].中国药学杂志,2015,(15):1292.
- [22] 陈俊,贾静,徐晓兰,等.基于COⅠ序列的紫河车药材及其混伪品的DNA条形码鉴定研究[J].中国中药杂志,2014,39(12):2204.
- [23] 刘旭朝,周丽思,刘金欣,等.基于COⅠ序列的水牛角及其易混伪品DNA条形码鉴定研究[J].药学学报,2017,52(3):494.
- [24] 刘晓帆,刘春生,杨瑶珺,等.基于COⅠ基因的龟甲及其混伪品的DNA条形码研究[J].中国中药杂志,2013,38(7):947.
- [25] 杜鹤,崔丽娜,姚辉,等.基于COⅠ条形码序列的珍珠母及其混伪品的DNA分子鉴定[J].中国现代中药,2011,32(11):176.
- [26] 杜鹤,崔丽娜,孙佳明,等.基于COⅠ序列的蛤壳及其混伪品的DNA分子鉴定[J].吉林中医药,2012,32(1):451.
- [27] 贾静,石林春,姚辉,等.市售动物药材僵蚕的DNA条形码鉴定研究[J].药学学报,2016,51(11):1784.
- [28] 杜鹤,孙佳明,崔丽娜,等.基于COⅠ条形码的麝香及其混伪品的DNA分子鉴定[J].吉林中医药,2011,31(5):451.
- [29] Erpenbeck D,Hooper J N,W?rheide G.COⅠ phylogenies in diploblasts and the Barcoding of Life′ — are we sequencing a suboptimal partition? [J].Mol Ecol Notes,2006,6(2):550.