夏伯候,熊苏慧,唐洁,张智敏,李亚梅,林丽美
XIA Bo-hou,XIONG Su-hui,TANG Jie,ZHANG Zhi-min,LI Ya-mei,LIN Li-mei

• 1:湖南中医药大学药学院湘产大宗药材品质评价湖南省重点实验室湖湘中药资源保护与利用协同创新中心

摘要(Abstract)：

夏枯草是一种多年生药食两用植物,具有丰富的抗氧化活性物质。该文研究了夏枯草水提取物乙酸乙酯和正丁醇萃取部位以及萃取剩余部位总黄酮,总酚酸,总三萜和总多糖含量以及它们的抗氧化能力(FRAP,DPPH和ABTS~+方法),并通过多元统计方法分析夏枯草多重药效物质与其抗氧化活性关系。结果表明,乙酸乙酯萃取部位具有相对较高含量的总黄酮、总酚酸和总三萜,分别为(0.61±0.10),(0.52±0.09),(0.21±0.03) g·g~(-1 )DW;同时,具有较高的清除DPPH和铁还原能力,分别为(3.1±0.38),(2.56±0.35) mmol·L~(-1)·g~(-1)DW。层次聚类分析和主成分分析的结果表明,这些化学成分与其抗氧化能力的综合信息可以代表不同部位的"差异性"。典型相关分析结果表明,夏枯草提取物多重药效物质与其抗氧化能力之间有较强的正相关性。其中,第一典型相关系数达到0.970 0。此外,ABTS~+方法对不同部位的药效物质响应较低,可能不适合于夏枯草得抗氧化活性评价,而总三萜和总酚酸更适合于DPPH的评价方法,该研究结果对夏枯草在食品或药品领域抗氧化活性的评价方式以及相关提取物的开发研究部具有重要的参考意义。
Prunellae Spica is a perennial edible and medicinal plant, rich in antioxidant substances. Total flavonoids(TFC), Phenolics(TPC), triterpenoids(TSC), polysaccharides(PC) and their antioxidant capacities(by the FRAP, DPPH and ABTS~+ methods) of ethyl acetate fraction, n-butanol fraction and other fractions of aqueous extract from Prunellae Spica were investigated in this study. Then the multivariate statistical method was adopted to analyze the relationship between the multiple pharmaceutical ingredients and antioxidant capacities of Prunellae Spica. The results showed that ethyl acetate fraction had relatively high concentration of TFC(0.61±0.10) g·g~(-1)DW, TPC(0.52±0.09) g·g~(-1)DW, and TSC(0.21±0.03) g·g~(-1)DW, with high scavenging capacity of DPPH(3.1±0.38) mmol·L~(-1)·g~(-1)DW and FRAP(2.56±0.35) mmol·L~(-1)·g~(-1)DW. Hierarchical clustering analysis(HCA) and principal component analysis(PCA) results indicated the information from chemical compositions and antioxidant capacity can represent the "differences" of different fractions. Canonical correlation analysis(CCorA) revealed a high positive correlation between the amounts of multiple chemical compositions and the antioxidant capacities(r=0.970 0), and the first canonical variate had been reached. Moreover, ABTS~+ method showed a low response to the compositions of different fractions, so this method may not be suitable for evaluation of Prunellae Spica antioxidant capacities, while DPPH evaluation method was more suitable for TSC and TPC. The results of this study have important reference significance for the evaluation method on antioxidant activity of Prunellae Spica in the field of food or medicine as well as for the development of related extracts.

关键词(KeyWords)： 夏枯草;抗氧化;层次聚类分析;主成分分析;典型相关分析
Prunellae Spica;antioxidant capacities;hierarchical clustering analysis;principal component analysis;canonical correlation analysis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81503041);; 国家“重大新药创制”科技重大专项(2013ZX09201019);; 湖南省自然科学基金项目(2017JJ4045);; 长沙市科技计划项目(kq1701073);; 湖湘青年科技创新创业平台项目(2013);; 湖南省十二五重点学科药学学科项目;; 湖南中医药大学人才引进项目(2014RS4009)

作者(Author): 夏伯候,熊苏慧,唐洁,张智敏,李亚梅,林丽美
XIA Bo-hou,XIONG Su-hui,TANG Jie,ZHANG Zhi-min,LI Ya-mei,LIN Li-mei

DOI: 10.19540/j.cnki.cjcmm.20181105.009

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